Proteome-wide Alterations in Escherichia coli Translation Rates upon Anaerobiosis
نویسندگان
چکیده
منابع مشابه
In vivo translation rates can substantially delay the cotranslational folding of the Escherichia coli cytosolic proteome.
A question of fundamental importance concerning protein folding in vivo is whether the kinetics of translation or the thermodynamics of the ribosome nascent chain (RNC) complex is the major determinant of cotranslational folding behavior. This is because translation rates can reduce the probability of cotranslational folding below that associated with arrested ribosomes, whose behavior is deter...
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متن کاملQuantitative analysis of the Escherichia coli proteome
Escherichia coli (strain ATCC 25922 in a stationary culture) cells were lysed with SDS and the lysates were processed according MED-FASP protocol. The released peptides were analyzed by LC-MS/MS. Protein content per bacterial cell was calculated on the basis of the DNA content. Absolute protein quantitation was performed using the 'Total Protein Approach'. The data are supplied in the article.
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ژورنال
عنوان ژورنال: Molecular & Cellular Proteomics
سال: 2010
ISSN: 1535-9476
DOI: 10.1074/mcp.m110.001826